Targeted Genomics for Pathogen and Microbial Communities
Hybridization capture is the most versatile technique for comprehensive, cost-effective sequencing of both viruses and bacteria in complex samples.
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Synthetic oligo in situ hybridization probes provide highly specific target detection compared to conventional genomic-derived alternatives
Presented at PAG 2023. myTags® Custom probes utilize sophisticated design to eliminate nonspecific elements that BAC-derived probes typically retain.
Comprehending Cornales: phylogenetic reconstruction of the order using the Angiosperms353 probe set
Premise Cornales is an order of flowering plants containing ecologically and horticulturally important families, including Cornaceae (dogwoods) and Hydrangeaceae (hydrangeas), among others. While many relationships in Cornales are strongly supported…
How does AVITI compare to Illumina?
While the output (FASTQ files) is the same, the sequencing chemistry is different. The AVITI uses “avidity sequencing,” which is a sequencing-by binding chemistry. Illumina sequencing employs a sequencing-by synthesis…
Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire
The Oxford Nanopore Technologies MinION™ sequencer is a small, portable, low cost device that is accessible to labs of all sizes and attractive for in-the-field sequencing experiments. Selective breeding of…
Optimizing Phylogenomics with Rapidly Evolving Long Exons: Comparison with Anchored Hybrid Enrichment and Ultraconserved Elements
Abstract. Marker selection has emerged as an important component of phylogenomic study design due to rising concerns of the effects of gene tree estimation err…
Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
…amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with…
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