…it strongly contributes to the construction of artificial cell-like systems. These systems, often called “synthetic cells”, can be used to model cellular processes, including membrane-centred ones. They are very innovative…
…avian radiation, Corvides (Crows and Jays, Birds-of-paradise, Vangas, and allies). We combined a genus-level data set of thousands of ultraconserved elements (UCEs) and a species-level, 12-gene Sanger sequence matrix to…
myTXTL requires high quality template DNA, which should be free of nucleases (DNases, RNases) and inhibitors of the TXTL machinery (e.g. EDTA, ethidium bromide, SDS, Cl- ions, ethanol). Preparation of…
…as the phages MS2, phix174 and T7. The reactions were treated according to standard procedures (trypsin treatment, cleaning) before performing liquid chromatography-mass spectrometry (LC-MS). Data analysis was performed using Sequest…
Gathering genomic-scale data efficiently is challenging for nonmodel species with large, complex genomes. Transcriptome sequencing is accessible for organisms with large genomes, and sequence capture probes can be designed from…
…Ectatomma, Gnamptogenys, Heteroponera, Holcoponera status revived, Poneracantha status revived, Rhytidoponera, Stictoponera status revived, and Typhlomyrmex. The new phylogenetic framework and classification proposed here will shed light on the study of…
…and purified expression components. For paperlike materials, we found that (1) materials based on synthetic polymers were mostly incompatible with cell-free expression, (2) lysate-based reactions were largely insensitive to the…
…Comprehensive – Pan-cancer panel targeting 1,000s of exons from 100s of genes Curated – Loci chosen from major cancer field publications Cost-effective – Lower per-reaction price than whole genome or…
…the expression dynamics, including the transcription dynamics, for the two synthetic cell free circuits. While, we considered only two circuits here, this approach could potentially be extended to simulate other…
…approaches to species delimitation based on target-enriched genomic data. With molecular data from thousands of ultraconserved elements (UCEs), mitochondrial genome sequences and morphometric data, I use an integrated approach to…
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