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myTags in situ Hybridization Probes – Applications and Services
Latest Webinar: myTags insitu Hybridization Probes – Applications and Services…
What (F)ISH protocol should I use with myTags Custom probes?
The latest recommended protocols for both labeled and immortal probe libraries can be found in the Resources tab, but in general, myTags FISH libraries are compatible with most FISH protocols….
What are options to analyze my samples after running myTXTL reactions when using the system for protein production?
…choose SDS-PAGE analysis, you can either take a small sample (1-3 µL) directly from your TXTL reaction, or – to reduce background signal – precipitate proteins with TCA/acetone or ammonium-acetate/methanol…
Multilocus molecular systematics of the circumtropical reef-fish genus Abudefduf (Pomacentridae): history, geography and ecology of speciation
We investigated a pantropical sub-family and genus of damselfishes, the sergeant-majors (Pomacentridae: Abudefdufinae: Abudefduf), to identify the tempo and mechanisms of speciation in the lineage. We examined sequence capture data…
myTXTL Sequences
P70 deGFP Control Description: Expresses deGFP from an E. coli P70a promoter (Cat. No. 502138), requires KL740 cells for propagation (ATCC Cat. No. BAA-3232) Plasmid: FASTA | GenBank T7 deGFP…
High phylogenetic utility of an ultraconserved element probe set designed for Arachnida
…the arachnid tree of life remain unresolved, hindering comparative approaches to arachnid biology. Biologists have made considerable efforts to resolve the arachnid phylogeny; yet, limited and challenging morphological characters, as…
Ancient proteins resolve controversy over the identity of Genyornis eggshell
…Genyornis newtoni (†Dromornithidae, Galloanseres) and were subsequently dated to before 50 ± 5 ka by Miller et al. [ Nat. Commun. 7, 10496 (2016)]. This was taken to represent the…
I want to express my protein, but need help with the DNA design. Are there qualified 5′ and 3′ regions (promoters, RBS, terminators) that have performed well in myTXTL?
…kit plasmid controls, to allow users to optimize their designs with respect to 5′ and 3′ sequences that enable optimal expression for their application. Please contact techsupport@arbor.daicel.com with any questions….
Parallel decay of vision genes in subterranean water beetles
…an assemblage of independently-evolved water beetle (Dytiscidae) species from a subterranean archipelago in Western Australia, where parallel vision and eye loss have occurred. Using a combination of transcriptomics and exon…
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