Overview
myTags Expert SARS-CoV-2 probes can detect full-length sequences of both the native viral RNA or positive (+) strand and the actively replicating RNA or negative (-) strand from the SARS-CoV-2 virus. These probe sets can be used in a variety of various FISH applications, including human and Vero cell cultures.
Designed from the complete sequenced SARS-CoV-2 genome using our advanced FISH probe design algorithms, these probes are highly specific to SARS-CoV-2 virus and are designed to not hybridize significantly to any other circulating human coronaviruses, including beta, alpha, and delta coronaviruses.
They are available in both immortal amplifiable format as well as ready-to-use fluorescent labeled format (3 label options) for maximum versatility in any (F)ISH experimental workflow.
Convenient—Predesigned for effectiveness, these probes are available in multiple configurations, including ready-to-use versions with multiple popular fluorophore options.
Versatile—Compatible with all cytogenomic applications for detection of native and actively replicating viral RNA.
Access to expertise—Our team of expert scientists provide complimentary project design assistance.
Easy to use—With ready-to-use labeled options and multiple optimized protocols available, myTags probes are ideal for new or expert cytogenetics ISH users.
myTags probes are for research use only and are not validated for diagnostic or therapeutic purposes.
Applications
myTags Expert SARS-CoV-2 probes are ideal for a range of research applications, including:
- Viral genome presence detection (ssRNA positive strand genomic RNA)
- Active viral replication detection (subgenomic negative strand RNA)
- Human cell lines and tissue
- Vero cell cultures
- Drug discovery research
myTags Expert SARS-CoV-2 probes are available in 2 configurations, to detect both the positive (+) strand and negative (-) strand sequences, and in two ready-to-use formats: immortal probes or labeled probes.
myTags probes are for research use only and are not validated for diagnostic or therapeutic purposes.
Performance
myTags Expert SARS-CoV-2 probes have been demonstrated as effective for the detection of SARS-CoV-2 in biological samples of clinical research relevance.
Highly specific detection of the SARS-CoV-2 viral genome Huh7 cells infected with SARS-CoV-2 and processed 2 days after exposure.
(A.) Detection of viral RNA genome using myTags SARS-CoV-2 (+) Strand Labeled Probe Set with ATTO 647N fluorescent tag (magenta). (B.) Detection of SARS- CoV-2 protein present in infected cells with immunocytochemistry (ICC) via antibodies labeled with Alexa 488 fluorescent tag (green). (C.) Signals merged, with cell nuclei visualized in gray/blue via DAPI staining. The co-localization of green and magenta signals demonstrates that the myTags SARS-CoV-2 Labeled Probe is highly specific at labeling cells with active SARS-CoV-2 viral infections. Learn more about this research project: Mirabelli et al. (2021), PNAS
myTags probes are for research use only and are not validated for diagnostic or therapeutic purposes.
Resources
myTags probes are for research use only and are not validated for diagnostic or therapeutic purposes.
FAQs
We recommend using the myTags labeling protocol with myTags immortal libraries for perfroming the labeling process in your own lab. If you would like to use a different protocol, one of our scientists would be happy to provide assistance to ensure success.
Probes are available in 2 configurations to detect both the positive (+) strand and negative (-) strand sequences:
Positive (+) strand probes– SARS-CoV-2 is a class IV RNA virus with an ssRNA genome (positive strand). The probes to detect the (+) strand will hybridize to full-length genomic (+) strand RNA as well as subgenomic (+) strand RNAs made during replication.
Negative (-) strand probes– Detection of the (-) strand is a hallmark of active viral replication. The probes to detect the (-) strand will hybridize to the full-length (-) strand as well as subgenomic (-) strand RNAs. In experimental applications where viral infection is not controlled, additional confirmation of SARS-CoV-2 presence via PCR is recommended.
NOTE: The positive (+) and negative (-) strand probes are not designed to be used in the same hybridization experiment. If co-hybridization is required for your experimental design, please contact us for a customized probe solution.
myTags Expert SARS-CoV-2 probes are available two ready-to-use formats:
- Immortal probes that are ready to be coupled with your preferred tag(s)
- Labeled probes with Alexa-488, ATTO-550, or ATTO-647N fluorescent tags. For experiments that need a brighter signal, the 3X fluorescent tag upgrade is recommended, which can aid detection of lower cellular viral RNA concentrations at earlier experimental time points.
myTags probes are for research use only and are not validated for diagnostic or therapeutic purposes.
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