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Comparison of Target-Capture and Restriction-Site Associated DNA Sequencing for Phylogenomics: A Test in Cardinalid Tanagers (Aves, Genus: Piranga)
…the UCE data. Additionally, in the UCE data, we analyzed a complete matrix as well as data sets with only highly informative loci. A complete matrix of 189 UCE loci…
Programmable On-Chip DNA Compartments as Artificial Cells
…cells capable of metabolism, programmable protein synthesis, and communication. Metabolism is maintained by continuous diffusion of nutrients and products through a thin capillary, connecting protein synthesis in the DNA compartment…
International Plant & Animal Genome 2022, Virtual. Focus: NGS
…methods, hybridization capture is the best technique for comprehensive, low-bias, and cost-effective genome or community sequencing of viruses and bacteria from complex samples. Here we review the principles of hybridization capture for…
Microbes and Metagenomics
…performance for both control mock community samples as well as biological samples. This myBaits panel was demonstrated to be an effective discovery tool not only enabling comprehensive ARG profiling but…
Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion
Mutations in mtDNA lead to muscular and neurological diseases and are linked to aging. The most frequent aberrancy is the “common deletion” that involves a 4,977-bp region flanked by 13-bp…
Detecting SARS-CoV-2 variants in wastewater and their correlation with circulating variants in the communities
…of coronaviruses circulating in the communities. Wastewater samples were collected from Truckee Meadows Water Reclamation Facility (TMWRF) from November 2020 to June 2021. SARS-CoV-2 variants resulting from wastewater were compared…
A draft genome and transcriptome of common milkweed ( Asclepias syriaca ) as resources for evolutionary, ecological, and molecular studies in milkweeds and Apocynaceae
…assembly of the common milkweed, Asclepias syriaca. This reconstruction of the nuclear genome is augmented by linkage group information, adding to existing chloroplast and mitochondrial genomic resources for this member…
International Plant & Animal Genome XXVIII in San Diego, CA. Focus: NGS
…sites, respectively. The broad range of adaptability of RGEN-ISL to different temperatures and combinations of methods has the potential to advance the field of chromosome biology. Complete Wheat Exome Analysis…
Cell-free microcompartmentalised transcription translation for the prototyping of synthetic communication networks (review)
Recent efforts in synthetic biology have shown the possibility of engineering distributed functions in populations of living cells, which requires the development of highly orthogonal, genetically encoded communication pathways. Cell-free…