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Do you have any examples of gene circuits that can be set up with myTXTL Toolbox 2.0 Collection plasmids?
Yes, please see examples in the Publications section; filter for myTXTL. Please note, that every gene circuit should start with a σ70-specific promoter like P70a….
Light in the darkness: New perspective on lanternfish relationships and classification using genomic and morphological data
…deep-sea fishes. Among deep-sea animals, the 257 species of lanternfishes (Myctophiformes) are among the most important open-ocean lineages, representing half of all mesopelagic vertebrate biomass. With this relative abundance, they…
Cell-Free Protein Systems from Yersinia pestis are Functional and Growth-Temperature Dependent
…°C lysate is comparable with the productivity of other non-E. coli systems. The data we present demonstrate that, without genetic modification to enhance productivity, cell-free extracts from Y. pestis are…
Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems
Cell-free transcription-translation (TXTL) systems produce RNAs and proteins from added DNA. By coupling their production to a biochemical assay, these biomolecules can be rapidly and scalably characterized without the need…
Expression Regulation by a Methyl-CpG Binding Domain in an E. coli Based, Cell-Free TX-TL System
…coli cell-free expression system, that MBD from the human transcriptional regulator MeCP2 performs as a specific, methylation-dependent repressor in conjunction with the BDNF (brain-derived neurotrophic factor) promoter sequence. Mutation of…
myTXTL Pro Kit for Cell-Free Protein Expression
Kit for industry-leading cell-free protein expression. Just add DNA to express analysis-ready proteins in hours, and avoid in vivo challenges….
Cell-Free DNA Detection of Tumor Mutations in Heterogeneous, Localized Prostate Cancer Via Targeted, Multiregion Sequencing
…localized prostate tumor tissue.METHODSLow-pass whole-genome and targeted sequencing with a machine-learning guided 2.5-Mb targeted panel were used to identify single nucleotide variants, small insertions and deletions (indels), and copy-number alterations…
myTXTL Cell-Free Expression of Antibodies and Antibody Fragments – PEGS 2024
The new myTXTL Antibody/DS Kit enables rapid, cell-free expression of antibody constructs….
myTXTL Antibody/DS Kit for Cell-Free Protein Expression
Cell-free expression of active antibodies and proteins with disulfide bonds. Just add DNA to express analysis-ready proteins in hours!…