myTXTL® is a versatile, easy-to-use cell-free expression platform for protein synthesis, enzyme engineering and synthetic biology in industrial and academic applications. Originally developed by Prof. Vincent Noireaux at the University of Minnesota, this revolutionary and powerful TX-TL technology was further refined at Daicel Arbor Biosciences to provide greater robustness and deliver reliable, consistent performance. This well-characterized technique has been employed in synthetic biology labs globally for a wide variety of applications including synthesis of toxic proteins, rapid enzyme engineering, and bacteriophage production.
myTXTL Sigma 70 Master Mix is the perfect choice for high-yield production of soluble and membrane proteins, rapid prototyping of gene networks using plasmid DNA, the construction of synthetic minimal cells and to study chemical synthesis. The myTXTL Linear DNA Expression Kit is engineered for efficient synthesis using linear DNA templates without the need for cloning and additional stabilizers. Simply add linear DNA template to the optimized master mix to begin protein synthesis. The myTXTL T7 Expression Kit has been designed for maximum robustness and reliable performance when using T7 promoter-driven constructs. Continuous co-expression of T7 RNA polymerase in the E. coli-based master mix drives protein production for any template with a T7 promoter. All options pair well with automated liquid handling systems, making it an ideal platform for rapid design-build-test cycles when screening libraries or analyzing genetic parts and gene circuits.
myTXTL unites an extremely user-friendly, safe GMO-free handling with exceptional protein synthesis efficiency and great versatility, which makes it similarly well-suited for cutting-edge research and student education. Check out our full line of myTXTL products below, or contact our expert scientists today to confer about which myTXTL product is best for your specific application.