Cell-Free Protein Expression
myTXTL – Cell-Free Expression
The myTXTL® system is a comprehensive solution for protein engineering and synthetic biology applications.

myTXTL® is a versatile, easy-to-use cell-free expression platform for protein synthesis, enzyme engineering and synthetic biology in industrial and academic applications. Originally developed by Prof. Vincent Noireaux  at the University of Minnesota, this revolutionary and powerful TX-TL technology was further refined at Arbor Biosciences to provide greater robustness and deliver reliable, consistent performance. This well-characterized technique has been employed in synthetic biology labs globally for a wide variety of applications including synthesis of toxic proteins, rapid enzyme engineering, and bacteriophage production.

myTXTL Sigma 70 Master Mix is the perfect choice for high-yield production of soluble and membrane proteins, rapid prototyping of gene networks using plasmid DNA, the construction of synthetic minimal cells and to study chemical synthesis. The myTXTL Linear DNA Expression Kit is engineered for efficient synthesis using linear DNA templates without the need for cloning and additional stabilizers. Simply add linear DNA template to the optimized master mix to begin protein synthesis. The myTXTL T7 Expression Kit  has been designed for maximum robustness and reliable performance when using T7 promoter-driven constructs. Continuous co-expression of T7 RNA polymerase in the E. coli-based master mix drives protein production for any template with a T7 promoter. All options pair well with automated liquid handling systems, making it an ideal platform for rapid design-build-test cycles when screening libraries or analyzing genetic parts and gene circuits.

myTXTL unites an extremely user-friendly, safe GMO-free handling with exceptional protein synthesis efficiency and great versatility, which makes it similarly well-suited for cutting-edge research and student education. Check out our full line of myTXTL products below, or contact our expert scientists today to confer about which myTXTL product is best for your specific application.

myTXTL T7 Expression Kit
Optimized for cell-free gene expression from T7-driven templates, either linear or circular DNA.
myTXTL Sigma 70 Master Mix Kit
A rapid, easy-to-use solution for protein expression in vitro, based on E. coli σ70 gene expression.
myTXTL Linear DNA Expression Kit
Optimized for cell-free protein synthesis from linear DNA template molecules.
myTXTL Toolbox 2.0 Plasmid Collection
The myTXTL Toolbox 2.0 Plasmid Collection is an excellent source for designing comprehensive gene circuits.
myTXTL CRISPR – Cell-Free Optimization
myTXTL CRISPR is a fast and easy-to-use solution for CRISPR analysis and optimization.


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