myTags are custom-designed using assembled sequences to address many of the drawbacks associated with Bacterial Artificial Chromosome (BAC) probes. The proprietary bioinformatics design process at Daicel Arbor Biosciences uses custom genomic coordinates from assembled sequences for identification of hundreds to thousands of highly-specific molecular probe sequences for hybridization only to the intended targets.

Unlike BAC probes, myTags synthetic probes collectively provide bright and reliable signal while minimizing or eliminating background signal associated with repetitive sequences and non-specific binding. Daicel Arbor Biosciences’s proprietary oligo synthesis technology provides parallel synthesis of tens of thousands of different probe sequences in a single pool, which dramatically reduces per-oligo synthesis cost and enables consistent batch manufacturing of any scale. Then the myTags Labeling Services can be applied to convert the synthetic oligos into fluorescently-labeled probes for immediate use.

Every myTags Custom order includes expert probe design assistance for a wide variety of cytogenomics applications. myTags ISH probes are available in both labeled, ready-to-use fluorescent formats, or as immortal probe pools that are ready to be amplified and labeled on demand following the myTags labeling protocol. Labeled probes are available in a wide range of popular tagging options, including fluorophores (see Product Sheet) and haptens, perfect for multiplexed (F)ISH.

myTags ISH probes have been successfully used across a diverse range of cytogenetic applications including 3D DNA-FISH, Cryo-FISH, RNA-FISH, Oligopaints, and chromosome painting for a variety of cell types. Contact our experts today to get started on your next myTags ISH project.