myBaits Expert — Predesigned Panels
myBaits Expert – Plant – Compositae-1061
This probe set (formerly named CompCOS) enables the rapid sequencing of hundreds of orthologous nuclear loci in the Daisy Family, Compositae (Asteraceae).

myBaits Expert – Plant – Compositae-1061

These probes have been demonstrated to enrich hundreds of single to low-copy protein-coding genes across members of Compositae, both at higher and lower taxonomic levels. Probes were designed from 1061 loci, each with 2 to 3 representative sequences from across the Daisy Family to maximize taxonomic breadth of the design (Mandel et al. 2014).

The baitset is broadly applicable for phylogenetic research across Compositae, as well as, members of its sister family Calyceraceae (Mandel et al. 2017). The baitset is available from Daicel Arbor Biosciences as an in-stock catalog kit available for immediate shipment at a low per-reaction cost. As with all myBaits kits, the Compositae-1061 probe set is provided as a complete solution target capture kit, including buffers, blockers, and baits, along with an easy-to-use protocol. Or if you would prefer to outsource the work, our myReads NGS service expert scientists are available to perform library preparation, target capture, and sequencing for your entire project.

  • Predesigned Panel – Designed by expert plant geneticists
  • Proven results – Demonstrated utility across broad taxonomic range
  • Freely accessible pipelines – Simple, user-friendly data analysis
  • Cost-effective – Use one bait set across multiple distantly-related taxa
Ordering Information
Size
Cat#
Qty
Price
myBaits COS Compositae 1Kv1
Kit for enrichment of 1,061 COS loci in the Compositae/Asteraceae plant family. Demonstrated to recover >700 loci across a range of Compositae species and closely-related families.
305208.v5
$830
Available in-stock for immediate shipment.
Please Note

This panel was previously described as “CompCOS 1Kv1” under the UCE line.



Overview

Introduction

This set of 9,678 120mer probes targets 1,061 conserved orthologous set (COS) loci in the Compositae/Asteraceae plant family. These ~10K probes are known to enrich >700 loci across a wide range of Compositae species as well as taxa from closely-related families. The bait list can be downloaded here.
It was first described in: JR Mandel, RB Dikow, VA Funk, RR Masalia, SE Staton, A Kozik, RW Michelmore, LH Rieseberg, JM Burke. 2014. A Target Enrichment Method for Gathering Phylogenetic Information from Hundreds of Loci: An Example from the Compositae. Applications in Plant Sciences

Hyb-Seq Compositae Tree of Life
Hyb-Seq Compositae Tree of Life.
Bayesian, concatenated species tree.
Performance


Hyb-Seq Compositae Tree of Life
Figure 2. Heat map of locus recovery efficiency in a variety of taxa with the Compositae-1061 probeset. Rows are samples and colums are genes. Colors indicate the percentage of the targeted locus length.
Resources

myBaits Kit Protocol

Current Version: v5

myBaits Manual v5
For kits shipped starting in Sept 2020, with Cat#’s ending in “.v5”

Visit the myBaits Manual archive page to download previous versions.

Safety Data Sheet

myBaits Kit SDS – US Version – v5 Chemistry – Sept 2020

myBaits Kit SDS – EU Version – v5 Chemistry – Sept 2020

Visit the SDS resources page to download previous versions.
Publications

Phylogenetic studies

Mandel, J.R., R.B. Dikow, C.M. Siniscalchi, R. Thapa, L.E. Watson, V.A. Funk. (2019). A fully resolved backbone phylogeny reveals numerous dispersals and explosive diversifications throughout the history of Asteraceae. Proceedings of the National Academy of Sciences.

Herrando-Moraira S., J.A. Calleja, P. Carnicero-Campmany, K.Fujikawa, M. Galbany-Casals, N. Garcia-Jacas, H-T. Im, S-C. Kim, J. Liu, J. López-Alvarado, J. López-Pujol, J.R. Mandel, S. Massó, I. Mehregan, N. Montes-Moreno, E. Pyak, C. Roquet, L. Sáez, A. Sennikov, A. Susanna, V. Roser. (2018). Exploring data processing strategies in NGS target enrichment to disentangle radiations in the tribe Cardueae (Compositae). Molecular Phylogenetics and Evolution.

Mandel, J.R., M.S. Barker, R.J. Bayer, R.B. Dikow, K. Jones, S. Keeley, C.M. Siniscalchi, R. Thapa, L.E. Watson, V.A. Funk. (2017). The Compositae Tree of Life in the age of phylogenomics. Journal of Systematics and Evolution.

Mandel, J.R., R.B. Dikow, V.A. Funk. (2015). Using phylogenomics to resolve mega-families: an example from Compositae. Journal of Systematics and Evolution.

Mandel, J. R., R. B. Dikow, V. A. Funk, R. R. Masalia, S. E. Staton, A. Kozik, R. W. Michelmore, L. H. Rieseberg, and J. M. Burke. (2014). A target enrichment method for gathering phylogenetic information from hundreds of loci: an example from the Compositae. Applications in Plant Sciences.

Microsatellite marker development

Thapa, R., R.J. Bayer, J.R. Mandel. (2019) Development and characterization of microsatellite markers for Antennaria corymbosa (Asteraceae) and close relatives. Applications in Plant Sciences, in press.

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