myBaits Expert — Predesigned Panels
myBaits Expert Whole Genome Enrichment (WGE)
Whole Genome Enrichment (WGE) is target capture of genome-wide DNA from a complex DNA source.

myBaits Expert Whole Genome Enrichment (WGE)

Daicel Arbor Biosciences’ WGE bait manufacturing technology can cost-effectively produce biotinylated RNA baits representative of an entire nuclear genome. This allows for bulk enrichment of genome-wide endogenous DNA from complex metagenomic samples, such as environmental or ancient DNA. To manufacture custom WGE baits, all that is required is a sample of high-quality genomic DNA from an organism of interest, or a close relative, even if the genome has not been sequenced.

Recovering appreciable full-genome coverage from metagenomic, environmental, or ancient DNA samples with only shotgun sequencing data is likely to be both expensive and bioinformatically intense. Standard and custom WGE kits from Daicel Arbor Biosciences can help overcome these challenges. Discover how myBaits WGE kits can dramatically increase the percentage of endogenous DNA reads from an NGS dataset.

Due to the high sequencing depth required to observe appreciable coverage of whole-genome data, especially from degraded or complex samples, Whole Genome Enrichment (WGE) kits are generally best suited for exploratory research projects. If the goal is to recover specific genes or markers of interest, then a focused custom myBaits panel is more appropriate for maximizing sequencing efficiency. If WGE is the proper application of choice, premade and custom WGE kits from Daicel Arbor Biosciences can help overcome many of these challenges.

  • Custom bait sets – Select from an organism of interest
  • Premade human baits – In stock and ready to ship for ancient DNA
  • Efficient genome-wide sequencing – Isolate genome of interest from complex samples
  • Cost-effective genomic bait manufacturing – Savings on baits and sequencing costs
  • Perfect for exploratory studies – Sequenced genome is not required
Ordering Information
myBaits WGE Human
Kit for human genome-wide enrichment, built from human genomic DNA. Caucasian, African, and Japanese options available.
myBaits WGE Custom
Kit for human genome-wide enrichment, built from custom user-supplied genomic DNA source.

Human WGE kits are in-stock for immediate shipment. Custom WGE kits typically require 2-4 weeks for production (starting from when Arbor receives a suitable genomic DNA sample).

What's Included?

Supplied as complete kit, including probes as well as all necessary reagents for the hybridization capture and wash steps.


Ordering Process

Daicel Arbor Biosciences has in-stock genomic DNA (gDNA) WGE panels for Human (Caucasian, African, or Japanese Male). To produce a Custom WGE panel, a high-purity, high-molecular weight gDNA sample is required to be shipped to Arbor’s manufacturing facility in Ann Arbor, MI, USA. You can ship this gDNA sample (lab-generated or commercially-sourced) to Arbor for processing, or for some organisms Arbor may be able to provide the additional service of acquiring the gDNA from a third-party vendor. Contact us if you have any questions about this process. Daicel Arbor Biosciences will then utilize our proprietary technology to transform the gDNA into ready-to-use genome-wide target capture baits.


Arbor Bio WGE Process Overview
Figure 1. Schematic demonstrating the production and typical application of myBaits WGE kits.

Baits can be produced from any pure eukaryotic or prokaryotic genomic DNA source, for example modern elephant. Daicel Arbor Biosciences’ unique process coverts this gDNA into a pool of biotinylated RNA baits. These baits can then enrich corresponding molecules from a user-supplied NGS library, for example from an ancient mammoth, via the process of myBaits in-solution hybridization capture.

This enriched library allows for orders-of-magnitude more efficient NGS than would be possible using the full, non-enriched library.


myBaits Kit Protocol

Current Version: v5

myBaits Manual v5
For kits shipped starting in Sept 2020, with Cat#’s ending in “.v5”
Please see tips in the manual PDF as well as FAQ tab for recommended procedure modifications for performing WGE from metagenomic/ancient DNA samples. In the v5 manual, following the “High Sensitivity” protocol is recommended. Visit the myBaits Manual archive page to download past versions.

Safety Data Sheet

myBaits Kit SDS – US Version – v5 Chemistry – Sept 2020

myBaits Kit SDS – EU Version – v5 Chemistry – Sept 2020

Visit the SDS resources page to download previous versions.
Can I input less than 100-500ng per capture reaction?

Target capture necessarily requires subjecting your libraries to a bottleneck, wherein target molecules are captured and enriched, and non-target molecules are therefore removed. To have sufficient unique molecules for good sequencing coverage of your targets, successful captures DEPEND on the input of sufficiently complex libraries. For this reason, for libraries with a significant non-target component (e.g., ancient, forensic, or environmental samples), and especially for WGE captures with a very large full nuclear genome target size, we strongly recommend maximizing the target component in each capture by using as much input library as possible (up to 2 µg+), and consider two rounds of capture for higher percentage of reads on-target.

For best results, it is recommended that only amplified (non-PCR-free) NGS libraries are used for target capture. This provides multiple copies of each starting template molecule, increasing the chance of each individual molecule getting enriched. However if you need more starting material to reach the recommended amount, it is generally preferable to generate more library from fresh genomic DNA or a new batch of indexed library, rather than through extra amplification. This is because while some amplification is good, over-amplification risks reducing the observable complexity of your libraries through the uneven action of PCR bias, as some molecules will become relatively more abundant while others become rare. This is also true for manipulating your libraries after capture: amplify your post-capture libraries the minimum number of cycles necessary to reach the molarity required by your sequencing facility.

Can I pool multiple samples in a single reaction?

We generally do NOT recommend pooling multiple samples per capture reaction for very degraded and/or rare targets (e.g. ancient DNA), or for very large targets (e.g. a WGE baitset targeting a full nuclear genome). In fact, for highly degraded samples, you may achieve better results by performing multiple parallel WGE reactions per sample.
There is a fully sequenced genome available for my organism – can you use this for WGE bait design?

The myBaits WGE product line ONLY offers bait production directly from high quality genomic DNA precursor material. This gDNA must be made physically available to us in order to manufacture a myBaits WGE Custom kit (either extracted in your lab or sourced from a third-party gDNA supplier).

Daicel Arbor Biosciences does have the ability to design and synthesize synthetic customized bait oligo pools via our standard myBaits Custom DNA-Seq, RNA-Seq, and Methyl-Seq products. However the myBaits Custom synthetic oligo approach is generally not an option for targeting entire large nuclear genomes (e.g. from eukaryotic organisms such as plants or animals) due to prohibitively large numbers of baits that would be required. However if you are working with an organism that has a much smaller genome (e.g. one or more bacteria), or are interested in capturing only a portion of a much larger genome, then a myBaits Custom kit is likely the most effective option.

Please contact us with details about your project goals and budget, so we can discuss project options.

Are there any constraints on the type of gDNA that Arbor can purchase for my Custom WGE kit?

To produce a Custom WGE panel, a high-purity, high-molecular weight genomic DNA (gDNA) sample is required to be shipped to Arbor’s manufacturing facility in Ann Arbor, MI, USA.

If you do not have access to the appropriate gDNA, for some organisms Arbor may be able to provide the additional service of acquiring the gDNA sample(s) from a third-party vendor.

Custom gDNA sourcing service is only upon prior arrangement and approval by Arbor. This service is only available for species whose genomic DNAs are available from vendors supplying the US. The gDNA sample(s) must be available for Arbor to purchase in sufficient quantity as a commercial entity without any special licenses, acquisition or use permits, or memberships.

Please contact us to provide specific details on the gDNA source(s)/species that you are interested in, and we will review the request to determine feasibility and pricing.

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