…wide range of applications, including: Phylogenomics Ultraconserved elements (UCEs) Anchored hybridization enrichment (AHE) Mitochondrial DNA (catalog or custom) Exon panels Custom & community panels Gene expression RNA-seq Metagenomics Environmental DNA…
…step. Cell-free transcription–translation (TX–TL) systems offer a simple and fast alternative to performing these characterizations in cells. Here we provide an overview of a cell-free TX–TL system that utilizes the…
…amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with…
…with a cell-free expression toolbox that works with the seven E. coli sigma factors. The E. coli endogenous holoenzyme E(70) is used as the primary transcription machinery. Elementary circuit motifs,…
Abstract. The bottom-up construction of biological entities from genetic information provides a broad range of opportunities to better understand fundamental p
…compared the performance of a target-capture approach using alternative sources of genomic DNA from ten specimens of spring salamanders (Plethodontidae: Gyrinophilus porphyriticus) collected between 1985 and 1990: allozyme supernatants, allozyme…
We study the liquid-liquid phase separation (LLPS) of a cell-free transcription-translation (TXTL) system. When the TXTL reaction, composed of a large amount of proteins, is concentrated, the uniformly mixed state…
…(Saccharum spp., Poaceae), which has a complex genome with a high level of ploidy and heterozygosity. In this study, we developed a set of oligonucleotide (oligo)-based probes through bioinformatic design…
No. myBaits hybridization capture kits have always been– and will continue to be– compatible with any user-provided upstream NGS library preparation system that is appropriate for a given project. However,…
…improve specificity, we aggressively filtered candidate repetitive sequences using a combination of nucleotide basic local alignment search tool (BLASTN) searches to the Triticeae Repetitive Sequence Database (TREP), identification of regions…
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