…on morphology-based phylogenies are conflicting, and the interspecific relationships remain incipient. We conducted the first molecular phylogeny of the family by analyzing sequence data from ultraconserved elements (UCEs) of the…
…proofreading impaired polymerase mutant (Y547S) of Marek’s disease virus that exhibits a hypermutator phenotype while maintaining unimpaired growth in vitro and wild-type (WT)-like pathogenicity in vivo. At the same time,…
…target capture & high-throughput sequencing, and working with difficult specimens. In order to facilitate transparent, collaborative projects, we have opted to not offer specific guarantees for target capture & sequencing…
…genetic regulation elements, simple genetic circuit motifs, protein variants or metabolic pathways. However, variability in cell-free expression systems is a known challenge, whether between individuals, laboratories, instruments, or batches of…
Nucleus, chromatin, and chromosome organization studies heavily rely on fluorescence microscopy imaging to elucidate the distribution and abundance of structural and regulatory components. Three-dimensional (3D) image stacks are a source…
…to study the border between chemistry and biology. So far, a fully synthetic cell has remained elusive, but chemists are progressing towards this goal by reconstructing cellular subsystems. Cell boundaries,…
Executing gene circuits by cell-free transcription−translation into cell-sized compartments, such as liposomes, is one of the major bottom-up approaches to building minimal cells. The dynamic synthesis and proper self-assembly of…
…S. lalandi population. The genetic diversity is slightly lower, but comparable in Chinese versus Australian yellowtail kingfish (Chinese population, expected heterozygosity: 0.19, observed heterozygosity: 0.19, nucleotide diversity: 0.19 ± 0.09;…
…genetic information stored in DNA can be expressed through transcription (TX) and translation (TL). The exploration of cell-sized confinement has been recently investigated by using micron-scale droplets and microfluidic devices….
…use of E. coli cell-free transcription-translation systems (TXTL) to vastly improve the speed and scalability of CRISPR characterization and validation. Unlike prior approaches that require protein purification or live cells,…
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