Overview

These easy-to-use NGS kits are designed to target genomes of pathogenic respiratory viruses, for use by virologists, epidemiologists, evolutionary biologists, and public health researchers.

Unlike other manufacturers, our solution provides full customization. Choose from our “precombined” option that includes all available viral bait modules, or build your own combination from our standalone panels that target individual viral groups.

The flexible nature of myBaits® can capture novel variants, including point mutations, small or large indels, or other genomic changes. Our kits are compatible with enriching both long and short NGS library molecules, even from degraded targets.

  • 21 bait sets for common human respiratory viruses, used individually or combined
  • Confirm existing variants or discover new genome variation
  • Easily detect and strain-type viruses in a single assay
  • Characterize tens to thousands of samples on a single sequencing run
  • Save orders of magnitude in sequencing costs of heavily host-contaminated samples
  • Compatible with degraded R/DNA e.g. wastewater, environmental samples, FFPE

Want data even faster? Leverage our expert myReads NGS services.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Performance

Comprehensive coverage with minimal sequencing

The myBaits Expert Respiratory Virus panels are effective both individually as well as in the combined set of probes which includes all modules.

Using NGS libraries built from combinations of low copy numbers of viral genomic material embedded in backgrounds of 50 ng human RNA or DNA, we observe high enrichment rates of three to six orders of magnitude, and comprehensive genome coverage closely matching the non-enriched source. In the data shown here, five mock samples with five different viruses (Influenza A, SARS-CoV-2, Rhinovirus, Roseola, and Measles) are enriched with their individual target modules as well as with the combined set of 21 modules, and compared with direct non-enriched shotgun sequencing.

Virus  Viral genome copies 

(+ 50 ng human) 

Fold enrichment µ  Genome coverage µ  Coverage depth µ  L.O.D. (copies) 
Influenza A  100,000  7.9K ×  99.7%  216.79  10 
1,000  123K ×  98.0%  7.94 
100  501K ×  75.4%  2.66 
10  1.4M ×  46.3%  1.42 
SARS-CoV-2  100,000  14K ×  99.7%  371.76  10 
1,000  98K ×  99.0%  8.01 
100  90K ×  61.5%  2.24 
10  1.4M ×  52.8%  1.55 
Rhinovirus  100,000  23K ×  98.7%  129.34  1,000 
1,000  76K ×  49.2%  1.97 
100  36K ×  8.7%  0.12 
10  82K ×  0.5%  1.55 
Roseola  100,000  1K ×  99.6%  158.93  100 
1,000  50K ×  97.6%  29.98 
100  60K ×  72.0%  2.37 
10  10K ×  7.7%  0.10 
Measles  100,000  1.8K ×  99.4%  95.59  100 
1,000  8.3K ×  48.3%  1.55 
100  13.7K ×  22.8%  0.33 
10  8.7K ×  2.9%  0.04 

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Publications

The myBaits Expert Respiratory Virus panel collection is composed of 21 distinct but combinable probesets for targeted NGS of whole virus genomes. We used hundreds of reference genomes for probe design to ensure that each module can retrieve a wide range of strains and variants.

* Shown in parentheses are number of reference genomes included in design for each module
Adenovirus (15+*)Metapneumovirus (6)
Bocavirus (3)Mumps (1)
Chickenpox (1)Orthopneumovirus / RSV (7)
Coronavirus Human (4)Parainfluenza (4)
Coronavirus Non-human (60)Parechovirus (6)
EBV (2)Rhinovirus (38)
Enterovirus (8)Roseola (4)
Influenza A (7)SARS (1)
Influenza B (1)SARS-CoV-2 (6)
Influenza C (1)+ Precombined version with all modules pre-mixed
Measles (1)
MERS (1)

To customize your myBaits Expert Respiratory Virus kit with individual modules, please complete the Panel Selection form [XLSX] and submit with your order.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Order Now

To customize your myBaits Expert Respiratory Virus kit with individual modules, please complete the Panel Selection form [XLSX] and submit with your order.

Ordering Information
Size
Cat#
Qty
Price
myBaits Expert Respiratory Virus Kit, with individual modules
Kit for enrichment of respiratory viral genomes. Must submit a Panel Selection form with order to specify individual bait module(s) from the list of 21 available panels. 8 reactions worth of baits are provided for each module/tube.
320108.V5
$$1,395.00
myBaits Expert Respiratory Virus Kit, precombined all-modules
Kit for enrichment of respiratory viral genomes, with all available target bait modules precombined.
322308.V5
$$2,675.00
Available in-stock for immediate shipment.

Our scientists are here to help launch your respiratory viral genome sequencing project with the myBaits Expert Respiratory Virus kit, or to design myBaits Custom kit for your specific research needs.

Need assistance?

Our scientists are ready to help you select the right Daicel Arbor Biosciences product for your research needs.

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