Rapidly sequence Ultraconserved Elements (UCEs) for phylogenetics research

Our myBaits® Expert UCE kits enable rapid, selective target enrichment of Ultraconserved Elements (UCEs). These genomic regions have been identified and exploited as ideal hybridization capture candidates for phylogenetic and population genetics research on novel and non-model species.

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Overview

UCEs are highly conserved genomic regions flanked by variable sequences used for phylogenetics. Sets of UCE loci have been identified for various taxonomic groups, including vertebrates, insects, and more. Our myBaits Expert UCE panels offer lower per-reaction costs and are in stock and available for immediate shipment.

Predesigned – Designed by experts and ready for immediate use.
Proven results – Ever-expanding list of peer-reviewed publications.
Analysis pipelines – Simple, user-friendly data analysis.
Cost-effective – Use one bait set across multiple distantly related taxa.

Want data even faster? Our expert myReads NGS services can process thousands of samples using UCE probe sets to move your project forward.

Additional info on the utility of UCEs for genetic research can be found at UltraConserved.org.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Available panels

Check back as we continue to expand our collection of UCE panels–or contact us to suggest a new addition.

Vertebrates

UCEs: Tetrapods 5K version 1

This set of 5,472 baits (120 mer) targets 5,060 UCEs from tetrapods/amniotes. The 5k probes are known to enrich ~4k loci in birds and reptiles, 2-3k loci in mammals, and up to 1k loci in amphibians. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, JE McCormack, NG Crawford, MG Harvey, RT Brumfield, TC Glenn. (2012.) Ultraconserved elements anchor thousands of genetic markers for target enrichment spanning multiple evolutionary timescales. Syst Biol

UCEs: Tetrapods 2.5K version 1

This set of 2,560 baits (120 mer) targets 2,386 UCEs from amniotes. The 2.5k probes are known to enrich ~2.2k loci in birds and reptiles and ~1k loci in mammals. These baits have not been tested extensively across all tetrapods. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, JE McCormack, NG Crawford, MG Harvey, RT Brumfield, TC Glenn. (2012) Ultraconserved elements anchor thousands of genetic markers for target enrichment spanning multiple evolutionary timescales. Syst Biol. This bait set has also been used for phylogeographic studies by BT Smith, MG Harvey, BC Faircloth, TC Glenn, RT Brumfield. (2013) Target capture and massively parallel sequencing of ultraconserved elements (UCEs) for comparative studies at shallow evolutionary time scales. Syst Biol

UCEs: Acanthomorph/Percomorph Fishes 1K version 1

This set of 2,628 baits (120 mer) targets 1,314 UCE loci present in Acanthomorph (Percomorph) fishes. The baits can be downloaded from https://ndownloader.figshare.com/files/11188235. The baitset was first described in: Alfaro ME, Faircloth BC, Harrington RC, Sorenson L, Friedman M, Thacker CE, Oliveros CH, Cerny D, Near TJ. (2018). Explosive diversification of marine fishes at the Cretaceous-Palaeogene boundary. Nature Ecology & Evolution

UCEs: Ostariophysan Fishes 2.7K version 1 - NEW!

This set of 6,737 baits (120 mer) targets 2,708 UCE loci present in Ostariophysan fishes. The baits can be downloaded from Figshare. The baitset was first described in: Faircloth BC, Alda F, Hoekzema K, Burns MD, Oliveira C, Albert JS, Melo BF, Ochoa LE, Roxo FF, Chakrabarty P, Sidlauskas BL, Alfaro ME (2018). A target enrichment bait set for studying relationships among ostariophysan fishes. BioRxiv.

UCEs: Ray-finned Fish (Actinopterygians) 0.5K version 1

This set of 2,001 baits (120 mer) targets 500 UCEs from fish. The 0.5k Actinopterygian probes are known to enrich 300-400 loci from ray-finned fishes. The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, Sorenson L, Santini F, Alfaro ME. (2013). A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). PLoS ONE

Invertebrates

UCEs: Arachnida 1.1K version 1

This set of 14,799 baits (120 mer) targets 1,120 UCEs from the Arachnida (spiders, mites, scorpions, harvestmen, and kin). The bait list can be downloaded here. It was first described in: BC Faircloth (2017.) Identifying Conserved Genomic Elements and Designing Universal Probe Sets To Enrich Them. Methods in Ecology and Evolution. The phylogenetic utility of this bait set was demonstrated in: J Starrett, S Derkarabetian, M Hedin, RW Bryson, JE McCormack, BC Faircloth. (2016.) High Phylogenetic Utility of an Ultraconserved Element Probe Set Designed for Arachnida. Mol Ecol Resources

UCEs: Spider 2K version 1

This set of 15,015 baits (120 mer) targets >2,000 UCE loci present in spiders (Araneae). The baits can be downloaded in the Supporting Information ZIP file available here. The baitset was first described in: Kulkarni S, H Wood, M Lloyd, G Hormiga. (2020). Spider‐specific probe set for ultraconserved elements offers new perspectives on the evolutionary history of spiders (Arachnida, Araneae). Molecular Ecology Resources 20: 185-203

UCEs: Insect Coleoptera 1.1K version 1

This set of 13,674 baits (120 mer) targets 1,172 UCEs from the Coleoptera (beetles). The bait list can be downloaded here. It was first described in: BC Faircloth (2017.) Identifying Conserved Genomic Elements and Designing Universal Probe Sets To Enrich Them. Methods in Ecology and Evolution.

UCEs: Insect Diptera 2.7K version 1

This set of 31,328 baits (120 mer) targets 2,711 UCEs from the Diptera (true flies). The bait list can be downloaded here. It was first described in: BC Faircloth (2017.) Identifying Conserved Genomic Elements and Designing Universal Probe Sets To Enrich Them. Methods in Ecology and Evolution.

UCEs: Insect Hemiptera 2.7K version 1

This set of 40,207 baits (120 mer) targets 2,731 UCEs from the insect order Hemiptera (true bugs). The bait list can be downloaded from Figshare. The bait sequences were first described in: Faircloth BC (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution. And first used in: Kieran TJ, Gordon ERL, Forthman M, Hoey-Chamberlain R, Kimball RT, Faircloth BC, Weirauch C, Glenn TC (2018). Insight from an Ultraconserved Element Bait Set Designed for Hemipteran Phylogenetics Integrated with Genomic Resources. Molecular Phylogenetics and Evolution.

UCEs: Insect Hymenoptera 2.5K version 2, Principal

An expanded version of the original HymV1 design, this set of 31,829 baits (120 mer) targets 2,590 UCE loci from the insect order Hymenoptera (ants, bees, sawflies, and wasps). The principal bait list can be downloaded from http://datadryad.org/resource/doi:10.5061/dryad.89n87. It was first described in: MG Branstetter, JT Longino, PS Ward, BC Faircloth. (2017). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. Methods Ecol Evol

UCEs: Insect Hymenoptera 2.5K version 2, Ant-Specific

This set of 9,898 baits (120 mer) targets 2,524 UCE loci present in Hymenoptera and 16 nuclear exons (12 genes) commonly sequenced in insects. The UCE baits are a subset of the more diverse “principal” bait set, including baits from ant genomes only. The exon baits were designed from four distantly related ant species. The UCE and exon bait lists can be downloaded from http://datadryad.org/resource/doi:10.5061/dryad.89n87. The baitset was first described in: MG Branstetter, JT Longino, PS Ward, BC Faircloth. (2017). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. Methods Ecol Evol

UCEs: Insect Hymenoptera 2.5K version 2, Ant-Bee-Specific

This set of 9,332 baits (120 mer) targets 2,545 UCE loci present in Hymenoptera and 12 nuclear exons (7 genes) commonly sequenced in insects. The UCE baits are a subset of the more diverse “principal” bait set, including baits from ant and bee genomes only. The baitset can be downloaded here. The baitset was first described in: Grab, H. et al. (2019) Agriculturally dominated landscapes reduce bee phylogenetic diversity and pollination services. Science 363(6424): 282-284.

UCEs: Insect Hymenoptera 1.5K version 1

This set of 2,749 baits (120 mer) targets 1,510 UCEs from the insect order Hymenoptera (ants, bees, sawflies, and wasps). The bait list can be downloaded from ultraconserved.org. It was first described in: BC Faircloth, MG Branstetter, ND White, SG Brady. (2015.) Target enrichment of ultraconserved elements from arthropods provides a genomic perspective on relationships among Hymenoptera. Mol Ecol Resources

UCEs: Insect Lepidoptera 1.3Kv1

This set of 14,363 baits (120 mer) targets 1,381 UCE loci present in Lepidoptera (butterflies, moths, and relatives). The baits can be downloaded from Figshare. The bait sequences were designed by and first described in: Faircloth BC (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Performance

Example alignment of a 120bp UCE locus from the Tetrapods 5K baitset compared to the region containing the top BLAST hit in genomes of chicken (Gallus gallus), human, mouse (Mus musculus), and frog (Xenopus laevis. Individual bases are colored by degree of similarity between sequences (dark = high, light = low). The central 120bp UCE region is strongly conserved across all taxa, whereas immediate flanking regions are more divergent

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.

Resources

Protocol Tip: We recommend reviewing the published UCE literature associated with your panel of choice to see how other researchers utilized their myBaits reagents, including factors such as library insert length, sequencing depth, hyb/wash temp, and more.