We offer a full suite of services for next-generation sequencing projects, on both regular specimens as well as degraded specimens such ancient DNA including:

  • Extraction of DNA and RNA from fresh or degraded samples
  • Short-insert DNA and RNA library preparation suitable for downstream sequencing with Illumina and similar platforms
  • Long-insert DNA library preparation suitable for downstream PacBio and Nanopore platforms
  • Target enrichment using myBaits catalog and custom kits
  • High-throughput sequencing on Illumina and PacBio instruments

Demonstration of a range of customizable NGS library insert size options available from NGS services team at Daicel Arbor Biosciences. Our experts can help you to choose the best average insert length appropriate for your specific myBaits target capture project, whether SNP resequencing, exon capture, UCE sequencing, or more. Depending on the quality of your DNA, the nature of your desired target regions, and your planned sequencing platform and read protocol, your project may benefit from either shorter or longer NGS library sizes.

Please find the current submission guidelines and sample preparation instructions in the “Resources” section. These guidelines should be sufficient for most standard DNA or RNA extracts derived from fresh, high-quality, relatively recent specimens (e.g. DNA extracted from fresh, frozen, or alcohol-preserved tissues) or for submission of pre-made NGS libraries.

In addition, we are currently accepting most service projects for atypical or sensitive samples, including specimens with degraded or rare targets (e.g., ancient DNA, museum, archival, metagenomic, environmental, etc). However, you should first contact us with more information about your samples & research goals, so that we can determine if we are able to accept your project.

Yes we can, however we caution that there is a higher risk of sample and/or target drop-out for low-input and/or low-quality samples. In our experience across many project types, the recommended amounts outlined in the Sample Preparation Guide provide the best results.

We have outlined specific recommendations for Standard, Degraded, and Ancient DNA handling in our Sample Preparation Guide. We may recommend or request that you change the handling/package type based on the specific quantity/quality of DNA present in your samples if they do not meet the criteria for the handling type that you ordered. If you decide to proceed with a handling/package type that does not align with the quantity/quality of the DNA, you assume the risk that your sample(s) may result in little or no useful data.

We do not have a minimum sample number, but we advise that smaller projects will have a higher per-sample cost than larger projects due to the labor required to process a batch of samples and our volume discount pricing. There is no maximum sample count per project.

Please see our NGS Service Policies PDF document (available under Resources) for more detailed information.

Due to the inherently variable nature of target capture & high-throughput sequencing experiments (especially for new, untested, custom baitsets), we are unable to offer service guarantees related to turnaround time, unique read sequencing coverage depth, on-target percentage, and other experimental outcomes.

In order to facilitate transparent, collaborative projects, we have opted to not offer specific guarantees for service projects, since every custom project is completely unique. In science, sometimes research projects do not go 100% according to plan, and for target capture, it is very difficult to plan for specific sequencing outcomes without prior experimental data. However, you will only pay for the services that we actually perform, and we will not accept a project before discussing all the options with you.

You will receive updates and estimated timelines for completion of different stages of your project. If you are working within a specific deadline, we will certainly make every effort to accommodate that, though we cannot guarantee that it will be possible to finish your project within that window.

We are able to expedite a limited number of projects at any one time, please inquire if you believe your project needs to be expedited. When a project is expedited, it will skip our regular queue. We do not guarantee a specific turnaround time guarantee for expedited projects.

Yes, you can provide libraries that you have made yourself. However, we would prefer that we make the libraries so that we have direct control over the uniformity and quality of the libraries going into target capture.

If you send us libraries, they must be compatible with dual-indexed 8bp index reads, and you must make us aware of any incompatibilities within your sample set before you receive a quote.

Please confer with us to make sure that we are fully aware of the type of libraries that you are using, since it is critical to choose the correct adapter-specific blocking oligos during capture and the right amplification primers after capture.

Yes, we are currently accepting sequencing-only projects on the AVITI platform. Libraries must be compatible with the AVITI platform – see FAQ “What kind of libraries are compatible with AVITI?” for more details.

Yes. We typically have enough material to perform multiple different types of captures from a single set of libraries that we generate. Depending on your specific bait set and sample configuration, it may be possible to pool more than one different bait design in a single capture reaction. Please contact us for more information about pricing for your individual project configuration needs.

We offer extraction services for DNA and RNA for multiple sample types. Some of the options for fresh samples are tissue (including plant leaves and seeds), cells, blood, and saliva. We also have the facilities and expertise for performing DNA extraction on degraded specimens, including bones, teeth, soft tissue, FFPE, and other sample types.

If you have special or sensitive samples, we may be able to offer extraction services for your project, depending on the circumstances. Please contact us directly to inquire about special service options.

Yes. We have a small cleanroom on-site where we perform all pre-PCR procedures. We can accommodate most common ancient, museum, or historical sample types, such as bones, teeth, and soft tissue. If you have a different or unusual sample type, please contact us to discuss your project needs in greater detail.