Yes, we can synthesize immortal probe libraries that can be labeled using the Oligopaints labeling method. Please note these probe libraries are not compatible with the standard myTags labeling protocol due to sequence requirements of the Oligopaints method.
We generally ask for up to 3-4 weeks after an order is placed to ship myTags libraries.
We recommend using the myTags labeling protocol with myTags immortal libraries for perfroming the labeling process in your own lab. If you would like to use a different protocol, one of our scientists would be happy to provide assistance to ensure success.
Probes are available in 2 configurations to detect both the positive (+) strand and negative (-) strand sequences:
Positive (+) strand probes– SARS-CoV-2 is a class IV RNA virus with an ssRNA genome (positive strand). The probes to detect the (+) strand will hybridize to full-length genomic (+) strand RNA as well as subgenomic (+) strand RNAs made during replication.
Negative (-) strand probes– Detection of the (-) strand is a hallmark of active viral replication. The probes to detect the (-) strand will hybridize to the full-length (-) strand as well as subgenomic (-) strand RNAs. In experimental applications where viral infection is not controlled, additional confirmation of SARS-CoV-2 presence via PCR is recommended.
NOTE: The positive (+) and negative (-) strand probes are not designed to be used in the same hybridization experiment. If co-hybridization is required for your experimental design, please contact us for a customized probe solution.
myTags Expert SARS-CoV-2 probes are available two ready-to-use formats:
- Immortal probes that are ready to be coupled with your preferred tag(s)
- Labeled probes with Alexa-488, ATTO-550, or ATTO-647N fluorescent tags. For experiments that need a brighter signal, the 3X fluorescent tag upgrade is recommended, which can aid detection of lower cellular viral RNA concentrations at earlier experimental time points.
Yes. Due to the manufacturing process, there might be a small pellet visible. It is critical that you resuspend this pellet back into the myTXTL Master Mix completely before aliquoting it to set up your myTXTL reaction(s).
Yes, myTXTL Linear DNA Expression Kit allows the use of both, linear and circular/plasmid templates.
Sample handling and storage is mainly determined by the stability of your molecule of interest (protein, DNA, RNA) and thus optimal conditions may need to be evaluated. But to ensure sample integrity, we would recommend to either process the myTXTL reaction immediately after performing the incubation or store it at ≤ -20 °C.
Limit the number of freeze-thaw-cycles as much as possible. Our studies have indicated that up to five freeze-thaw-cycles do not negatively influence protein production efficiency of the myTXTL Master Mix when using flash freezing in liquid nitrogen and subsequent -80C storage.
Yes, please see examples in the Publications section; filter for myTXTL. Please note, that every gene circuit should start with a σ70-specific promoter like P70a.
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